Influence of Rotational Nucleosome Positioning on Transcription Start Site Selection in Animal Promoters
نویسندگان
چکیده
منابع مشابه
Influence of Rotational Nucleosome Positioning on Transcription Start Site Selection in Animal Promoters
The recruitment of RNA-Pol-II to the transcription start site (TSS) is an important step in gene regulation in all organisms. Core promoter elements (CPE) are conserved sequence motifs that guide Pol-II to the TSS by interacting with specific transcription factors (TFs). However, only a minority of animal promoters contains CPEs. It is still unknown how Pol-II selects the TSS in their absence. ...
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The DNA sequence determinants which direct RNA Pol-II to the correct transcription start site (TSS) are only partly understood. Conserved DNA motifs (core promoter elements) or a conserved nucleosome architecture may play a role in TSS selection. A complicating factor is that promoters are quite variable in many respects. Some have very focused while others have highly dispersed initiation site...
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Nucleosome depletion at transcription start sites (TSS) has been documented genome-wide in multiple eukaryotic organisms. However, the mechanisms that mediate this nucleosome depletion and its functional impact on transcription remain largely unknown. We have studied these issues at human MHC class II (MHCII) genes. Activation-induced nucleosome free regions (NFR) encompassing the TSS were obse...
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During transcription initiation, RNA polymerase (RNAP) binds to promoter DNA, unwinds promoter DNA to form an RNAP-promoter open complex (RPo) containing a single-stranded 'transcription bubble,' and selects a transcription start site (TSS). TSS selection occurs at different positions within the promoter region, depending on promoter sequence and initiating-substrate concentration. Variability ...
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ژورنال
عنوان ژورنال: PLOS Computational Biology
سال: 2016
ISSN: 1553-7358
DOI: 10.1371/journal.pcbi.1005144